Here are questions that I recall from my exam. The wording is approximate, but the content of the questions and answers is essentially the same as the real exam. I’ve taken liberties with the incorrect choices, as I don’t recall each one exactly as it appeared. Good luck!
1. Procedure #1 detected 50/100 true positives and 100/100 true negatives
Procedure #2 detected 80/100 true positives and 70/100 true negatives
a. procedure 1 is more sensitive
b. procedure 2 is more sensitive
c. procedure 1 is more sensitive and specific
d. procedure 2 is more sensitive and specific
2. TP/TP+FN = ?
3. What might the following indicate?
urine: RBCs, WBCs, nitrite, bacteria
c. nephrotic syndrome
d. renal calculi
4. Why is albumin the first protein to be detected in tests for renal failure?
a. its molecular size is largest
b. its molecular size is smallest
c. it is very negatively charged
5. Cortisol excess will result in _____
c. elevated glucose levels in blood
d. decreased glucose levels in blood
6. What is the reason for the following discrepancy?
Patient cells vs anti-A demonstrate a reaction strength of 3+
Patient cells vs anti-B demonstrate a reaction strength of 3+
Patient serum vs reagent A cells demonstrate a reaction strength of 3+
Patient serum vs reagent B cells demonstrate a reaction strength of 0
a. This patient demonstrates depressed anti-B production due to old age
b. This patient has multiple myeloma
c. This patient may be type A2B
d. The 3+ reaction against reagent A cells is due to dirty glassware
7. This spiral-form organism is seen in urine and cultured on Fletcher’s media
8. Organism that gives off a bleach-like odor in culture?
9. Presence of rheumatoid factor in blood may result in false positives for what test?
10. Disease associated with the following results? Elevated TSH; Elevated T3; Elevated free T4
c. pituitary tumor
11. If excess PTH is released, what would you find in elevated amounts in serum?
12. Mucoid, pink colonies on plate; produces gas; indole (+). On TSI tube you see yellow on the slant and yellow in the deep. What organism is this?
b. E. coli
c. Klebsiella pneumonia
d. Klebsiella oxytoca
13. PAD (+); indole (+); Organism stains gram negative. What is it?
a. P. vulgaris
b. P. mirabilis
14. You see a curved gram negative bacilli. It was cultured from the GI tract of a person with ulcers. What test would you do next to confirm its identity?
a. test for Urease
b. culture the organism in agar
c. H2S test
15. Enzyme controls run on a machine give results around -3 standard deviations. Samples run on the same machine give results of less than 1 standard deviation. What could be the problem?
a. controls are recently expired
b. controls were left at room temp for several days
16. HIV-1 & HIV-2 combination ELISA test is positive in a patient with symptoms of immune deficiency. Western blot was inconclusive for HIV-1. What do you do next?
a. rerun western blot for HIV-1
b. do a CD4 cell count
c. do HIV-2 ELISA
d. do HIV-2 western blot
17. What are the steps of PCR?
a. transduction, transcription, annealing
b. annealing, denaturation, transcription
c. denaturation, annealing, transcription
18. RAST test detects what?
a. IgE to particular antigens
19. After collecting a blood sample in an EDTA tube for CBC, you find that the Hematocrit is very high (67%). What should you do next?
a. collect blood again, but use less sodium citrate in the tube
b. collect blood in heparin
c. report these results
20. When you conduct a procedure using fluorescence, it’s important to protect yourself from the:
a. cover light
b. emitted light
c. exciting light
21. Blood was collected on Nov 1. Blood was then frozen in glycerol on Nov 5. What should the expiration date read?
a. Nov 1; 1 year from now
b. Nov 5; 1 year from now
c. Nov 1; 10 years from now
d. Nov 5, 10 years from now
22. A person was successfully treated for syphilis 12 years ago. However, he has just come in again, worried about having been re-infected. What would you look for in his blood?
23. A patient demonstrates a positive antibody screen. You suspect either Jka, K or c antibodies. You know from a previous history that this patient has Jka antigen on their red cells. You then react the patients serum with cells positive for certain antigens and see the following:
Patient serum vs: reagent K cells reagent c cells
Reaction strength: 0 4+
What can you conclude about the antigenic makeup of this patients red cells?
a. confirm patient as having K and c antigens on their red cells
b. rule out c antigen on the patients cells and confirm K antigen on their cells
c. rule out c and K antigens on patients red cells
d. rule out c antigen but cannot confirm the presence or absence of K antigen on the patients red cells
24. Urine protein chemistry dipstick (Reagent strip) detected no proteins but sulfosalicylic acid (SSA) test did detect proteins. Why?
a. reagent strip was expired
b. Bence-Jones proteins in urine
25. I was shown a picture of what I believe were several immature granulocytes in a peripheral blood smear. What stain should you use next to figure out this persons problem?
a. specific esterase
b. non specific esterase
26. Syndrome of inappropriate antidiuretic hormone secretion (SIADH) would result in what in blood?
a. excess potassium
b. excess sodium
c. excess non-serum water (?something like that?)
d. deficient potassium
e. deficient sodium
27. Fiber strands in urine resemble what under the microscope?
a. waxy cast
b. hyaline cast
c. WBC cast
d. fine granular cast
28. Which of the following regulates myocyte contraction?
b. cardiac troponins
29. HBa1c levels cannot always be used to monitor glucose levels in conditions such as:
a. sickle cell disease