Learning bacteriology can be daunting if you don’t know WHAT to focus on. Fortunately, studying for the ASCP is a very practical matter. In a real laboratory, the organism would arrive in some type of media. You would ask yourself, “I wonder what this organism is?” You would then culture and gram stain it, or perform some other tests to figure out what you’re dealing with.
Each organism produces a unique combination of results based on certain testing criteria. In many laboratories, all of the tests can be done very quickly on a single strip of reagents (API), which change colors based on different enzymatic properties of the organism. However, it’s not practical to memorize positive or negative for every test, for every type of organism. I found that using flowcharts – as if I were conducting manual laboratory testing – made things easier for me.
These are the flowcharts I produced. Once I had these, there was very little additional work to be done. I simply did questions to solidify my recall of the information on the charts.
An excellent microbiology professor suggested using tables to see what’s unique about a single organism, as it can help you memorize what is so similar about the others. For example, referring to the table of Gram Negative Diplococci, notice how moraxella catarrhalis is negative for acid production from oxidation of glucose, maltose and lactose. Also notice how only one organism is superoxol or DNase positive. By memorizing these few unique characteristics, you’ve essentially memorized all the others!
Unfortunately, you just have to memorize some things. For example, here are a few must-know properties of the (H)ACEK organisms
This is probably a simple concept if you’ve learned about it in lab or at school. If you haven’t, it can be frustrating trying to understand it on your own. I went through quite a few resources on the internet before I finally found a good explanation – and a light bulb went on in my head. I hope this picture can serve you similarly
These are the parasites I had to know for school. Only one showed up on my licensing exam so this is decidedly NOT high-yield, although it may help during coursework. I would definitely urge you to know the similarities/differences between the four major Plasmodium species (number of merozoites per schizont; shape, etc)