How to solve an antibody panel
This is how you can solve the simple type of panel you’ll encounter on the ASCP exam. Swipe through the 10 slides, or tap one to open it full size.
More blood bank charts
Antibody characteristics
The note about Le is VERY high yield. In fact, the entire card describing antibody characteristics is important. Undoubtedly, you will have at least one question based on characteristics of antibodies.
The Le (Lewis) system
Lewis antigens aren't produced by the red cell. They're adsorbed onto it from the plasma.
| If the Le gene is inherited | the person has Lea adsorbed onto RBCs | Le(a+b−) |
| If the Le and Se genes are inherited | the person has Leb adsorbed onto RBCs | Le(a−b+) |
| Characteristic | Antibodies |
|---|---|
| Naturally occurring | ABO Lewis P1 MN Lua |
| ★ Clinically significant | ABO Rh Kell Duffy Kidd SsU |
| ★ Warm antibodies | Rh Kell Duffy Kidd |
| Cold antibodies | M N P1 |
| ★ Usually only react in AHG | Kell Duffy Kiddnot detected on IS |
| Can react in any phase of testing | Lewis |
| ★ Detection enhanced by enzyme treatment of test cells | Rh Lewis Kidd |
| ★ Not detected with enzyme treatment of test cells | M N Duffy |
| Enhanced by acidification | M |
| Show dosage | Rh other than D MNS Duffy Kidd |
| Bind complement | I Kidd Lewis |
| Cause in vitro hemolysis | ABO Lewis Kidd Vel some P1 |
| Labile in vivo and in vitro | Kidd |
| Common cause of anamnestic response (delayed transfusion reaction) | KiddKidds are delayed |
| ★ Associated with paroxysmal cold hemoglobinuria (PCH) | Anti-PIgG |
★ = rows I starred · Kell, Duffy, Kidd keep showing up together: clinically significant, warm, and usually only react in AHG · AHG = antihuman globulin · IS = immediate spin · Se = secretor gene · PCH = paroxysmal cold hemoglobinuria (anti-P is the Donath-Landsteiner antibody)
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